Ki-67 polyclonal antibody

Product Specifications

Background

Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase) . It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index) . In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

CAS Number

9007-83-4

Structure Composition

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Product Name Alternative

Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_MOUSE.

Swiss Prot

Q91VE6

Reactivity

Human, Mouse

Immunogen

KLH conjugated synthetic peptide derived from mouse Ki-67:1231-1330/2938

Conjugation

Unconjugated

Applications

IHC-P

Dilution

IHC-P=1:100-500 () not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.

Purity

Affinity purified by Protein A

Modification

Unmodification

Molecular Weight

~358kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Ki-67 polyclonal Antibody detects endogenous levels of Ki-67 protein.

Applications Notes

Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.