Phosphotyrosine monoclonal antibody
Product Specifications
Background
The phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research. Antibodies that specifically recognize phosphorylated tyrosine residues have proved to be invaluable to the study of tyrosine phosphorylated proteins and the biochemical pathways in which they function.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
Phospho-tyrosine; Phospho tyrosine; Phospho-tyr; Phospho tyr; p-Tyr; p-tyrosine
Reactivity
Human, Mouse, Rat, Phosphotyrosine
Immunogen
KLH conjugated Phosphotyrosine
Conjugation
Unconjugated
Applications
IHC-P
Dilution
IHC-P=1:100-500
Purity
Affinity purified by Protein G
Modification
Phosphorylation
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Phosphotyrosine Monoclonal Antibody detects endogenous levels of Phosphotyrosine protein.
Applications Notes
Paraformaldehyde-fixed, paraffin embedded (Rat liver) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phosphotyrosine) Monoclonal Antibody, Unconjugated at 1:400 overnight at 4°C.
Host or Source
Mouse
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