TAP-Tag monoclonal antibody
Product Specifications
Background
Tandem affinity purification (TAP) is a purification technique for studying protein–protein interactions. It involves creating a fusion protein with a designed piece, the TAP tag, on the end. The original TAP method involves the fusion of the TAP tag to the C-terminus of the protein under study. The TAP tag consists of calmodulin binding peptide (CBP) from the N-terminal, followed by tobacco etch virus protease (TEV protease) cleavage site and Protein A, which binds tightly to IgG. The relative order of the modules of the tag is important because Protein A needs to be at the extreme end of the fusion protein so that the entire complex can be retrieved using an IgG matrix.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
TAP Tag; TAPTag; Tandem affinity purification
Reactivity
TAP-Tag
Immunogen
Recombinant TAP-Tag
Conjugation
Unconjugated
Applications
WB
Dilution
WB=1:1000-5000
Purity
Affinity purified by Protein G
Modification
Unmodification
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
TAP-tag monoclonal antibody detects over-expressed or recombinant proteins containing the TAP epitope tag.
Applications Notes
Primary: Anti-TAP-Tag at 1/1000 dilution
Host or Source
Mouse
Available Sizes
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