AKIRIN2 Polyclonal Antibody

Product Specifications

Background

Required for the innate immune response. Downstream effector of the Toll-like receptor (TLR), TNF and IL-1 beta signaling pathways leading to the production of IL-6. Forms a complex with YWHAB that acts to repress transcription of DUSP1.

CAS Number

9007-83-4

Structure Composition

0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Product Name Alternative

AKIR2_HUMAN; AKIRIN 2; Akirin-2; Akirin2; C6orf166; dJ486L4.2; FBI1; FLJ10342; Fourteen three three beta interactant 1.

Swiss Prot

Q53H80

Reactivity

Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep

Immunogen

KLH conjugated synthetic peptide derived from human AKIRIN2:51-150/203

Conjugation

Unconjugated

Applications

IHC-P

Dilution

IHC-P=1:100-500

Purity

Affinity purified by Protein A

Modification

Unmodification

Molecular Weight

22kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

AKIRIN2 Polyclonal Antibody detects endogenous levels of AKIRIN2 protein.

Applications Notes

Paraformaldehyde-fixed, paraffin embedded (Mouse brain) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AKIRIN2) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.