Phospho-CK II beta (Ser209) Polyclonal Antibody
Product Specifications
Background
This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
Casein Kinase 2 beta (phospho S209) ; Casein Kinase 2 beta (p-S209) ; p-CSK2B (Ser209) ; phospho-CSK2B (Ser209) ; Casein kinase 2 beta polypeptide; casein kinase IIβ; Casein kinase II subunit beta; CK II beta; CK2B; CK2N; CSK2B; CSK2B_HUMAN; CSNK 2B; CSNK2B; G5A; MGC138222; MGC138224; Phosvitin; Protein G5a.
Swiss Prot
P67871
Reactivity
Human, Mouse, Rat, Pig, Cow
Immunogen
KLH conjugated synthesised phosphopeptide derived from human CK II beta around the phosphorylation site of Ser209:FK (p-S) PV
Conjugation
Unconjugated
Applications
IHC-P
Dilution
IHC-P=1:100-500
Purity
Affinity purified by Protein A
Modification
Phosphorylation
Molecular Weight
24kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Phospho-CK II beta (Ser209) Polyclonal Antibody detects endogenous levels of CK II beta protein only when phosphorylated at S209.
Applications Notes
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK II beta (Ser209) ) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.
Host or Source
Rabbit
Available Sizes
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