JAK1 (phospho-Y1022) polyclonal antibody

Product Specifications

Background

JAK1 (Janus kinase 1) belongs to the family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK1. JAK1 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. Upon ligand binding, JAK1 undergoes tyrosine phosphorylation and catalytic activation in an interdependent manner. Phosphorylation of tyrosine residues at positions 1022 and 1023 is believed to function in the activation of catalytic events.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Tyrosine-protein kinase JAK1; Janus kinase 1; JAK-1; JAK1; JAK1A; JAK1B

Swiss Prot

P23458

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human JAK1 around the phosphorylation site of Tyrosine 1022.

Conjugation

Unconjugated

Related Pathways

Jak Stat : IL-6 Receptor Signaling, Inhibition of Apoptosis

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 125 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

P-JAK1 (Y1022) polyclonal antibody detects endogenous levels of JAK1 protein only when phosphorylated at Tyr1022.

Applications Notes

Western blot (WB) analysis of JAK1 (phospho-Y1022) polyclonal antibody at 1:500 dilution LaneA:HEK293T whole cell lysate (40ug) LaneB:AD293 whole cell lysate (40ug) LaneC:Hela whole cell lysate (40ug)