What is the catalog number for His-tag (4C2) monoclonal antibody?

The catalog number for His-tag (4C2) monoclonal antibody is AP0032M.

What sizes are available for His-tag (4C2) monoclonal antibody?

His-tag (4C2) monoclonal antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does His-tag (4C2) monoclonal antibody come in?

His-tag (4C2) monoclonal antibody is available in 2 different pack sizes.

Is His-tag (4C2) monoclonal antibody in stock?

His-tag (4C2) monoclonal antibody is currently made to order / not held in immediate stock. Contact us for lead time.

His-tag (4C2) monoclonal antibody

Product Specifications

Background

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.

CAS Number

9007-83-4

Structure Composition

1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

Reactivity

Transfected

Immunogen

Synthetic peptide His-tag.

Conjugation

Unconjugated

Applications

WB IP IF

Dilution

WB:1:5000~10000 IP:1:50~100 IF:1:50~200

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

His-tag (4C2) mAb detects over-expressed or recombinant proteins containing the 6xHis epitope tag.

Applications Notes

Western blot (WB) analysis of His-tag mAb at 1:5000 dillution Lane1:HEK293F whole cell lysate Lane2:HEK293F whole cell lysate, transfected with pcDNA3.4-Flag-N-RBD-His.