His-tag (4C2) monoclonal antibody
Product Specifications
Background
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. A system that has proven to be very successful relies on the insertion of a six histidine (His6) sequence in the N-terminus of the encoded protein, allowing for efficient coupling to Ni2+-chelating resins and purification by single step affinity chromatography. This polyhistidine sequence can then be removed by specific cleavage at sites recognized by enzymes such as thrombin or enterokinase, permitting the separation of the target protein from the polyhistidine tag.
CAS Number
9007-83-4
Structure Composition
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag
Reactivity
Transfected
Immunogen
Synthetic peptide His-tag.
Conjugation
Unconjugated
Applications
WB IP IF
Dilution
WB:1:5000~10000 IP:1:50~100 IF:1:50~200
Purity
The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
His-tag (4C2) mAb detects over-expressed or recombinant proteins containing the 6xHis epitope tag.
Applications Notes
Western blot (WB) analysis of His-tag mAb at 1:5000 dillution Lane1:HEK293F whole cell lysate Lane2:HEK293F whole cell lysate, transfected with pcDNA3.4-Flag-N-RBD-His.
Host or Source
Mouse
Available Sizes
Frequently Asked Questions
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