WNT7B polyclonal antibody
Product Specifications
Background
This gene is a member of the WNT gene family, which consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Among members of the human WNT family, this gene product is most similar to WNT7A protein.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
Protein Wnt-7b; Wingless related MMTV integration site 7B; Wingless type MMTV integration site family member 7B; WNT7B; WNT7B_HUMAN
Swiss Prot
P56706
Reactivity
Human, Rat
Immunogen
KLH conjugated synthetic peptide derived from human WNT7B:241-300/349
Conjugation
Unconjugated
Applications
WB ELISA IHC IF
Dilution
WB 1:500-2000 ELISA 1:5000-10000 IHC 1:100-500 IF 1:100-500
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 36 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
WNT7B polyclonal antibody detects endogenous levels of WNT7B protein.
Applications Notes
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-WNT7B Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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