PRICKLE2 polyclonal antibody
Product Specifications
Background
This gene encodes a homolog of Drosophila prickle. The exact function of this gene is not known, however, studies in mice suggest that it may be involved in seizure prevention. Mutations in this gene are associated with progressive myoclonic epilepsy type 5.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
PRIC2; PRIC2_HUMAN; prickle-like 2 (Drosophila) ; prickle-like 2; Prickle-like protein 2; PRICKLE2
Swiss Prot
Q7Z3G6
Reactivity
Mouse
Immunogen
KLH conjugated synthetic peptide derived from human Prickle 2:401-500/844
Conjugation
Unconjugated
Applications
IHC ICC IF
Dilution
IHC 1:100-500 ICC 1:100-500 IF 1:100-500
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 96 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PRICKLE2 polyclonal antibody detects endogenous levels of PRICKLE2 protein.
Applications Notes
Paraformaldehyde-fixed, paraffin embedded (Mouse brain) ; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Prickle 2) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C.
Host or Source
Rabbit
Available Sizes
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