ATG12 (A98) polyclonal antibody

Product Specifications

Background

Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ubiquitin-like protein ATG12; Autophagy-related protein 12; APG12-like; ATG12; APG12; APG12L

Swiss Prot

O94817

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to Human ATG12.

Conjugation

Unconjugated

Related Pathways

Autophagy Signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 20, 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ATG12 (A98) polyclonal antibody detects endogenous levels of ATG12 protein.

Applications Notes

Western blot (WB) analysis of ATG12 (A98) polyclonal antibody at 1:500 dilution Lane1:SP2/0 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:SHSY5Y whole cell lysate (40ug) Lane5:HCT116 whole cell lysate (40ug)