ATG12 (S129) polyclonal antibody

Product Specifications

Background

Atg12 (autophagy-related protein 12), also known as APG12, APG12L, FBR93 or HAPG12, is a 140 amino acid protein that is ubiquitously expressed and belongs to the Atg12 family of proteins. Atg12 is a homolog of the yeast protein Apg12 that participates in autophagy. Autophagy is a membrane trafficking mechanism that delivers cytoplasmic cargo to the vacuole/lysosome for degradation and recycling.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Ubiquitin-like protein ATG12; Autophagy-related protein 12; APG12-like; ATG12; APG12; APG12L

Swiss Prot

O94817

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human ATG12.

Conjugation

Unconjugated

Related Pathways

Autophagy Signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 18, 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

ATG12 (S129) polyclonal antibody detects endogenous levels of ATG12 protein.

Applications Notes

Western blot (WB) analysis of ATG12 (S129) polyclonal antibody at 1:500 dilution Lane1:C6 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug)