TR4 polyclonal antibody

Product Specifications

Background

Testicular receptor 4 (TR4), also called TAK1 or NR2C2, is an orphan receptor in a distinct subclass of the steroid hormone nuclear receptor superfamily along with TR2. TR4 forms heterodimers with TR2 and binds to DNA elements containing direct repeats (DRs) . TR4 plays a role in various processes, including neurogenesis, spermatogenesis, RNA metabolism, and protein translation. TR4 can interact with other hormone receptors such as AR and ER to influence protein-protein binding and target gene inactivation. High expression of TR4 correlates with prostate cancer metastasis and invasion through downstream targets such as CCL2 and Ezh2. Expression of TR4 in other cancers such as NSCLC and testicular germ cell tumors has also been associated with poor prognosis.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Nuclear receptor subfamily 2 group C member 2; Orphan nuclear receptor TAK1; Orphan nuclear receptor TR4; Testicular receptor 4; NR2C2; TAK1, TR4

Swiss Prot

P49116

Reactivity

Human, Pig

Immunogen

Synthetic peptide, corresponding to Human TR4.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:2000~1:5000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 67 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TR4 polyclonal antibody detects endogenous levels of TR4 protein.

Applications Notes

Western blot (WB) analysis of TR4 polyclonal antibody at 1:2000 dilution Lane1:The Lung tissue lysate of Pig (40ug) Lane2:The Spleen tissue lysate of Pig (40ug) Lane3:The Brain tissue lysate of Pig (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug)