PAK1/2/3 (E417) polyclonal antibody

Product Specifications

Background

Three isoforms of serine/threonine kinases, designated αPAK p68, βPAK p65 and γPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The α, β and γPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on γPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with γPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Serine/threonine-protein kinase PAK 1; Alpha-PAK; p21-activated kinase 1; PAK-1; p65-PAK; PAK1; PAKα; Serine/threonine-protein kinase PAK 2; Gamma-PAK; PAK65; S6/H4 kinase; p21-activated kinase 2; PAK-2; p58; PAK-2p27; PAK-2p34; C-t-PAK2; PAK2; PAKγ; Serine/threonine-protein kinase PAK 3; Beta-PAK; Oligophrenin-3; p21-activated kinase 3; PAK-3; PAK3; OPHN3; PAKβ

Swiss Prot

Q13153/Q13177/O75914

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human PAK1/2/3.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics, ErbB HER Signaling, MAPK Erk in Growth and Differentiation Pathway (, Regulation of Microtubule Dynamics, TGFβ Smad signaling

Applications

WB IF

Dilution

WB: 1:1000~1:2000 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 61 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

PAK1/2/3 (E417) polyclonal antibody detects endogenous levels of total PAK protein.

Applications Notes

Western blot (WB) analysis of PAK1/2/3 (E417) polyclonal antibody at 1:1000 dilution Lane1:SSGC7901 whole cell lysate (40ug) Lane2:U-87MG whole cell lysate (40ug) Lane3:THP-1 whole cell lysate (40ug) Lane4:RAW264.7 whole cell lysate (40ug) Lane5:C6 whole cell lysate (40ug)