SQSTM1 polyclonal antibody

Product Specifications

Background

The chronic focal skeletal disorder, Paget’s disease of bone, affects 2-3% of the population over the age of 60 years. Paget’s disease is characterized by increased bone resorption by osteoclasts, followed by abundant new bone formation that is of poor quality. The disease leads to several complications including bone pain and deformities, as well as fissures and fractures. Mutations in the ubiquitin-associated (UBA) domain of the Sequestosome 1 protein (SQSTM1), also designated p62 or ZIP, commonly cause Paget’s disease since the UBA is necessary for aggregate sequestration and cell survival.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Sequestosome-1; EBI3-associated protein of 60 kDa; EBIAP; p60; Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa; Ubiquitin-binding protein p62; SQSTM1; ORCA; OSIL

Swiss Prot

Q13501

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length Human SQSTM1.

Conjugation

Unconjugated

Related Pathways

Autophagy Signaling

Applications

WB IHC IP IF

Dilution

WB: 1:500~1:1000 IF: 1:50~1:200 IHC: 1:50~1:200 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 60 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

SQSTM1 polyclonal antibody detects endogenous levels of SQSTM1 protein.

Applications Notes

Western blot (WB) analysis of SQSTM1 polyclonal antibody at 1:500 dillution Lane1:Hela whole cell lysate (40μg) Lane2:NIH-3T3 whole cell lysate (30μg) Lane3:PC12 whole cell lysate (40μg)