TRα polyclonal antibody
Product Specifications
Background
Thyroid hormone nuclear receptors (TRs) are ligand-dependent transcription factors which regulate and control many metabolic and developmental processes. There are two genes encoding TRs identified to date, TRα and TRβ. TRs bind to thyroid hormone response elements (TREs) with half-site binding motifs in the orientation of palindromes, direct repeats or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3'-triiodo-L-thyronine (T3) . Transcriptional regulation by TRs is also modulated by heterodimerization with TR nuclear accessory proteins, the most extensively characterized of which are the retinoid X receptors (RXRα, RXRβ and RXRγ) . The TRα isoform TRα1 can display both a nuclear and undefined cytoplasmic location, and is the only TR that is imported into the mitochondrial matrix. TRα2 is a C-terminal variant of TRα1 that does not bind thyroid hormones (THs) and weakly binds DNA. TRα2 acts as a dominant negative antagonist of TH signalling.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Thyroid hormone receptor alpha; Nuclear receptor subfamily 1 group A member 1; V-erbA-related protein 7; EAR-7; c-erbA-1; c-erbA-alpha
Swiss Prot
P10827
Reactivity
Human, Pig
Immunogen
Synthetic peptide, corresponding to Human TRα.
Conjugation
Unconjugated
Applications
WB IF
Dilution
WB: 1:2000~1:5000 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 58 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
TRα polyclonal antibody detects endogenous levels of TRα protein.
Applications Notes
Western blot (WB) analysis of TRα polyclonal antibody at 1:5000 dilution Lane1:THP-1 whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:The Kidney tissue lysate of Pig (40ug) Lane4:The Liver tissue lysate of Pig (40ug)
Host or Source
Rabbit
Available Sizes
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