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IkB-α (S36) polyclonal antibody

Product Specifications

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3

Swiss Prot

P25963

Reactivity

Human, Mouse

Immunogen

Synthetic peptide, corresponding to Human IkB-α.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, Death Receptor Signaling, MAPK Erk in Growth and Differentiation Pathway (, Inhibition of Apoptosis, NF-kB Signaling, Phospholipase Signaling, T cell receptor signaling, Toll-like Receptor signaling

Applications

WB IF

Dilution

WB: 1:1000~1:2000 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95%

Modification

Unmodification

Molecular Weight

~ 39 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

IkB-α (S36) polyclonal antibody detects endogenous levels of IkB-α protein.

Applications Notes

Western blot (WB) analysis of IkB-α (S36) polyclonal antibody at 1:1000 dilution Lane1:MEF whole cell lysate (40ug) Lane2:MCF-7 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug)

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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