RPS6 (L234) polyclonal antibody
Product Specifications
Background
One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation. Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions. These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression, as well as ribosomal proteins and elongation factors necessary for translation. Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
40S ribosomal protein S6; Phosphoprotein NP33; Small ribosomal subunit protein Es6; RPS6; OK/SW-cl.2
Swiss Prot
P62753
Reactivity
Human, Rat, Mouse
Immunogen
Synthetic peptide, corresponding to Human RPS6.
Conjugation
Unconjugated
Related Pathways
MAPK Erk in Growth and Differentiation Pathway (, PI3K AKT signaling, Translational Contral elF4 and p70 S6 Kinase
Applications
WB IP
Dilution
WB: 1:5000~1:10000 IP: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 32 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
RPS6 (L234) polyclonal antibody detects endogenous levels of RPS6 protein.
Applications Notes
Western blot (WB) analysis of RPS6 (L234) pAb at 1:5000 dilution Lane1:AML-12 whole cell lysate (40ug) Lane2:The Liver tissue lysate of Mouse (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:Jurket whole cell lysate (40ug) Lane5:THP-1 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Frequently Asked Questions
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