CYP2E1 polyclonal antibody

Product Specifications

Background

Cytochrome P450 2E1, metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Product Name Alternative

Cytochrome P450 2E1; 4-nitrophenol 2-hydroxylase; CYPIIE1; Cytochrome P450-J; CYP2E1; CYP2E

Swiss Prot

P05181

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human CYP2E1.

Conjugation

Unconjugated

Applications

Dilution

WB: 1:1000~1:2000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

CYP2E1 polyclonal antibody detects endogenous levels of CYP2E1 protein.

Applications Notes

Western blot (WB) analysis of CYP2E1 polyclonal antibody at 1:1000 dilution Lane1:Pancl whole cell lysate (40ug) Lane2:A375 whole cell lysate (40ug) Lane3:LO2 whole cell lysate (40ug) Lane4:HEK293T whole cell lysate (40ug) Lane5:A549 whole cell lysate (40ug)