DAX1 polyclonal antibody

Product Specifications

Background

DSS-AHC critical region on the X chromosome protein 1 (DAX1) is an orphan nuclear receptor encoded by the nuclear receptor subfamily 0 group B member 1 (NR0B1) gene. DAX1 possesses an atypical DNA binding domain that allows it to form heterodimeric complexes with DNA binding partners and repress transcriptional activity. During development, DAX1 is important for establishment of the hypothalamic-pituitary-adrenal gonadal axis. The receptor is essential for development of several important hormone-producing organs that determine this axis, including the adrenal glands, pituitary, hypothalamus, and the male and female reproductive organs. Research studies suggest that DAX1 plays a role in maintenance of pluripotency in embryonic stem cells. Loss of DAX1 function through deletion or mutation results in adrenal insufficiency and hypogonadotropic hypogonadism, while duplication of the NR0B1 gene on the X-chromosome causes dosage-sensitive sex reversal.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Nuclear receptor subfamily 0 group B member 1; DSS-AHC critical region on the X chromosome protein 1; Nuclear receptor DAX-1

Swiss Prot

P51843

Reactivity

Human, Pig

Immunogen

Synthetic peptide, corresponding to Human DAX1.

Conjugation

Unconjugated

Applications

WB IF IHC

Dilution

WB: 1:1000~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 48 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

DAX1 polyclonal antibody detects endogenous levels of DAX1 protein.

Applications Notes

Western blot (WB) analysis of DAX1 polyclonal antibody at 1:2000 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:The Kidney tissue lysate of Pig (40ug)