NURR1 polyclonal antibody
Product Specifications
Background
Nurr1 (Nur-related factor 1) and Nur77 (also designated NGFI-B) encodeorphan nuclear receptors which may comprise an additional subfamily withinthe nuclear receptor superfamily. The rat and human homologs of mouse Nurr1are designated RNR1 and NOT, respectively. Both Nurr1 and Nur77 are growthfactor inducible, immediate early response genes. Induction of both Nurr1 andNur77 is seen after membrane depolarization while only Nur77 induction isseen with NGF stimulation. JunD acts as a mediator for Nur77. An increase in Nurr77 expression is seen in activated T cells during G0 to G1 transition andthroughout the G1 phase. In addition to its function as an immediate earlygene, Nur77 may play a role in TCR-mediated apoptosis. Cyclosporin A, apotent immuno-suppressant, has been shown to inhibit the ability of Nur77to bind DNA. A dominant negative form of Nur77 can protect T cell hybridomas from activation-induced apoptosis. However, the absolute requirementof Nur77 for TCR-mediated apoptosis is still under debate.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Product Name Alternative
Nuclear receptor subfamily 4 group A member 2; Immediate-early response protein NOT; Orphan nuclear receptor NURR1; Transcriptionally-inducible nuclear receptor; NR4A2; NOT; NURR1; TINUR
Swiss Prot
P43354
Reactivity
Human
Immunogen
Synthetic peptide, corresponding to Human NURR1.
Conjugation
Unconjugated
Applications
WB IHC
Dilution
WB: 1:2000~1:5000 IHC: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 75 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
NURR1 polyclonal antibody detects endogenous levels of NURR1 protein.
Applications Notes
Western blot (WB) analysis of NURR1 polyclonal antibody at 1:2000 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
Curated Selection
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