AR polyclonal antibody

Product Specifications

Background

Androgen receptor (AR), a zinc finger transcription factor belonging to the nuclear receptor superfamily, is activated by phosphorylation and dimerization upon ligand binding. This promotes nuclear localization and binding of AR to androgen response elements in androgen target genes. Research studies have shown that AR plays a crucial role in several stages of male development and the progression of prostate cancer.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Product Name Alternative

Androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4; AR; DHTR; NR3C4

Swiss Prot

P10275

Reactivity

Human, Mouse

Immunogen

Synthetic peptide, corresponding to Human AR.

Conjugation

Unconjugated

Applications

WB IF IHC

Dilution

WB: 1:2000~1:5000 IHC: 1:50~1:200 IF: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 140 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

AR polyclonal antibody detects endogenous levels of AR protein.

Applications Notes

Western blot (WB) analysis of AR polyclonal antibody at 1:2000 dilution Lane1:CT-26 whole cell lysate (40ug) Lane2:PC3 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug)