Raptor (S792) polyclonal antibody

Product Specifications

Background

Regulatory associated protein of FRAP, also designated Raptor, is a binding partner for mammalian target of rapamycin kinase (FRAP), and is essential for FRAP signalling in vivo. Raptor binding to FRAP is critical for FRAP-catalysed substrate phosphorylation of 4E-BP1. The raptor-FRAP complex is nutrient-sensitive and is important for a mechanism by which cells coordinate cell growth and size with changing environmental conditions. Raptor serves as a negative regulator of FRAP kinase activity under nutrient-deprived conditions and is an important component in the FRAP pathway. Raptor is highly expressed in skeletal muscle and to a lesser extent in brain, kidney, lung and placenta.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Regulatory-associated protein of mTOR; Raptor; p150 target of rapamycin (TOR) -scaffold protein; RPTOR; KIAA1303; RAPTOR

Swiss Prot

Q8N122

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding Human Raptor.

Conjugation

Unconjugated

Related Pathways

Phosphoinosotode Signaling, PI3K AKT signaling, Insulin &Glucose Signaling, Glutamine Metabolism, MTOR signaling

Applications

WB IHC

Dilution

WB: 1:500~1:1000 IHC: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 150 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Raptor (S792) polyclonal antibody detects endogenous levels of Raptor protein.

Applications Notes

Western blot (WB) analysis of Raptor (Ser792) polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug)