IKB-α (S32/S36) polyclonal antibody

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins. Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB. IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified.

NF-kappa-B inhibitor alpha; I-kappa-B-alpha; IkB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein MAD3; NFKBIA; IKBA; MAD4; NFKBI

Regulation of Apoptosis, Death Receptor Signaling, MAPK Erk in Growth and Differentiation Pathway (, Inhibition of Apoptosis, NF-kB Signaling, Phospholipase Signaling, T cell receptor signaling, Toll-like Receptor signaling

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Western blot (WB) analysis of IKB-α (S32/S36) polyclonal antibody at 1:5000 dilution Lane1:CT-26 whole cell lysate (40ug) Lane2:PC12 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:HepG2 whole cell lysate (40ug) Lane5:Hela whole cell lysate (40ug)