MEK1 (Phospho-S298) polyclonal antibody
Product Specifications
Background
MEK1 (Mitogen activated protein kinase kinase 1) catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. MEK1 activates ERK1 and ERK2 MAP kinases. Mitogen activated protein kinase kinase 2 (MEK2 or MAPKK2) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1 and 2 and MAPK enzymes by phosphorylating both residues within the threonine/glutamate/tyrosine (TEY) motif in the activation loop. MEK1 and 2 are also activated by dual phosphorylation, which occurs on serine 218 and 222, in the activation loop of the MEK. Threonine 292 of MEK1 is phosphorylated by ERK 2, which serves as a negative feedback loop by suppressing activation of MEK1.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Dual specificity mitogen-activated protein kinase kinase 1; MAP kinase kinase 1; MAPKK 1; MKK1; ERK activator kinase 1; MAPK/ERK kinase 1; MEK 1; MAP2K1; MEK-1; PRKMK1
Swiss Prot
Q02750
Reactivity
Human, Rat, Mouse
Immunogen
Synthetic phosphopeptide derived from human MEK1 around the phosphorylation site of Serine 298.
Conjugation
Unconjugated
Related Pathways
Regulation of Actin Dynamics, Warburg Effect, G Protein-coupled Receptors Signaling to MAPK Erk, MAPK Erk in Growth and Differentiation Pathway, MAPK Erk in Growth and Differentiation Pathway
Applications
WB IF IHC
Dilution
WB: 1:1000~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 45 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
MEK1 (Phospho-S298) polyclonal antibody detects endogenous levels of MEK1 protein only when phosphorylated at Ser298.
Applications Notes
Western blot (WB) analysis of MEK1 (Phospho-S298) polyclonal antibody at 1:1000 dilution Lane1:PMVEC whole cell lysate (40ug) Lane2:BV2 whole cell lysate (40ug) Lane3:Hela treated with λ-phosphatase whole cell lysate (40ug) Lane4:HeLa whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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