C-IAP1 polyclonal antibody

Product Specifications

Background

The inhibitor of apoptosis protein (IAP) family consists of an evolutionarily conserved group of apoptosis inhibitors containing a conserved 70 amino acid BIR (baculovirus inhibitor repeat) domain. Human members of this family include c-IAP1, c-IAP2, XIAP, survivin, livin, and NAIP. Overexpression of IAP family members, particularly survivin and livin, in cancer cell lines and primary tumors suggests an important role for these proteins in cancer progression. In general, the IAP proteins function through direct interactions to inhibit the activity of several caspases, including caspase-3, caspase-7, and caspase-9. In addition, binding of IAP family members to the mitochondrial protein Smac blocks their interaction with caspase-9, thereby allowing the processing and activation of the caspase.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Baculoviral IAP repeat-containing protein 2; Cellular inhibitor of apoptosis; C-IAP1; hIAP-2; Hiap2; RING finger protein 48; RING-type E3 ubiquitin transferase BIRC2; TNFR2-TRAF-signaling complex protein 2

Swiss Prot

Q13490

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to Human c-IAP1.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, Necrotic Cell Death, Death Receptor Signaling

Applications

Dilution

WB: 1:2000~1:5000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 62 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

C-IAP1 polyclonal antibody detects endogenous levels of c-IAP1 protein.

Applications Notes

Western blot (WB) analysis of TGFβ c-IAP1 polyclonal antibody at 1:2000 dilution Lane1:HCT116 whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:HepG2 whole cell lysate (40ug) Lane4:PC3 whole cell lysate (40ug) Lane5:A549 whole cell lysate (40ug)