TGFβ1 (Y369) polyclonal antibody

Product Specifications

Background

Transforming growth factor betas (TGFβs) were originally discovered due to their ability to promote anchorage-independent growth of rat NRK fibroblasts in the presence of TGFα. It is now realized that TGFβs mediate many cell-cell interactions that occur during embryonic development. Three TGFβs have been identified in mammals. TGFβ1, TGFβ2 and TGFβ3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecules.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Transforming growth factor beta-1; TGF-beta-1; Latency-associated peptide; LAP; TGFB1; TGFB; TGF β1

Swiss Prot

P01137

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to Human TGFβ1.

Conjugation

Unconjugated

Related Pathways

Tumor Angiogenesis, Contribution of Soluble Factors to EMT, TGFβ Smad signaling

Applications

Dilution

WB: 1:5000~1:10000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

TGF β1 (Y369) polyclonal antibody detects endogenous levels of TGF β1 protein.

Applications Notes

Western blot (WB) analysis of TGFβ1 (Y369) pAb at 1:5000 dilution Lane1:C6 whole cell lysate (40ug) Lane2:BV2 whole cell lysate (40ug) Lane3:A549 whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug)