PPARG (Phospho-T166) polyclonal antibody
Product Specifications
Background
Peroxisome proliferator-activated receptor γ (PPARγ) is a member of the ligand-activated nuclear receptor superfamily and functions as a transcriptional activator. PPARγ is preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Besides its role in mediating adipogenesis and lipid metabolism, PPARγ also modulates insulin sensitivity, cell proliferation and inflammation. PPARγ transcriptional activity is inhibited by MAP kinase phosphorylation of PPARγ at Ser84.
CAS Number
9007-83-4
Structure Composition
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.
Product Name Alternative
Peroxisome proliferator-activated receptor gamma; Short name=PPAR-gamma; Nuclear receptor subfamily 1 group C member 3; PPARG; NR1C3; PPAR γ; PPARγ
Swiss Prot
P37231
Reactivity
Human
Immunogen
Synthetic phosphopeptide derived from human PPARG around the phosphorylation site of Threonine 166.
Conjugation
Unconjugated
Related Pathways
Nuclear Receptor Signaling, MTOR signaling
Applications
WB
Dilution
WB: 1:1000~1:2000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Phosphorylation
Molecular Weight
~ 60 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
PPARG (Phospho-T166) polyclonal antibody detects endogenous levels of PPARG protein only when phosphorylated at Thr166.
Applications Notes
Western blot (WB) analysis of PPARG (Phospho-T166) polyclonal antibody at 1:1000 dilution Lane1:PC3 whole cell lysate (40ug) Lane2:MCF-7 treated with TNF-alpha (20 ng/ml,10 minutes) and Calyculin A (100 nM,10 minutes) whole cell lysate (40ug) Lane3:MCF-7 whole cell lysate (40ug)
Host or Source
Rabbit
Available Sizes
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