GYS (P539) polyclonal antibody

Product Specifications

Background

Glycogen is a polysaccharide of glucose and serves as an energy storage in mammalian muscle and liver. Glycogen synthase catalyzes the rate-limiting step of glycogen biosynthesis and has two major isoforms in mammals -- muscle isoform (GYS1) and liver isoform (GYS2) respectively. Glycogen synthase kinase-3α (GSK-3α) and glycogen synthase kinase-3β (GSK-3β) phosphorylate glycogen synthase at multiple sites in its C-terminus (Ser641, Ser645, Ser649 and Ser653) inhibiting its activity. Hypoxia alters glycogen metabolism including temporal changes of GYS1 expression and phosphorylation in cancer cells, suggesting the role of metabolic reprogramming of glycogen metabolism in cancer growth.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

Glycogen [starch] synthase, muscle; GYS1; GYS

Swiss Prot

P13807

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human GYS.

Conjugation

Unconjugated

Related Pathways

Regulation of Actin Dynamics, Insulin &Glucose Signaling

Applications

Dilution

WB: 1:500~1:1000

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 84 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

GYS (P539) polyclonal antibody detects endogenous levels of GYS protein.

Applications Notes

Western blot (WB) analysis of GYS (P539) polyclonal antibody at 1:500 dilution Lane1:H1792 whole cell lysate (40ug) Lane2:LO2 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug)