CD3e monoclonal antibody

Product Specifications

Background

When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery . This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) . Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and the p85 subunit of PI-3 kinase . TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck .

CAS Number

9007-83-4

Structure Composition

Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Product Name Alternative

T3E; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e

Swiss Prot

P07766

Reactivity

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within human CD3e. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

T cell receptor signaling

Applications

WB, IHC

Dilution

WB (1/500 - 1/1000), IHC (1/100 - 1/300)

Purity

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .