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Cytokeratin 18 monoclonal antibody

Product Specifications

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases.

CAS Number

9007-83-4

Structure Composition

Mouse IgG2a. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Product Name Alternative

CYK18; Keratin type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

Swiss Prot

P05783

Reactivity

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within human Cytokeratin 18. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB, IHC, IF

Dilution

WB (1/500 - 1/1000), IHC (1/100 - 1/300), IF (1/100 - 1/500)

Purity

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 50 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of Cytokeratin 18 protein.

Applications Notes

Western blot analysis of Cytokeratin 18 expression in MCF7 (A) whole cell lysates.

Host or Source

Mouse

Available Sizes

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