STAT3 monoclonal antibody

Product Specifications

Background

Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3b appears to be activated by both while Stat3a is activated by EGF, but not by IL-6. Highest expresion of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by Prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

APRF; Signal transducer and activator of transcription 3; Acute-phase response factor

Swiss Prot

P40763

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein corresponding to human STAT3.

Conjugation

Unconjugated

Related Pathways

Immune Cell Signaling, Jak Stat : IL-6 Receptor Signaling

Applications

WB, IHC

Dilution

WB (1/1000 - 1/2000), IHC (1/100 - 1/200)

Purity

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .