PARP1 monoclonal antibody
Product Specifications
Background
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) . PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.
CAS Number
9007-83-4
Structure Composition
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
ADPRT; PPOL; Poly [ADP-ribose] polymerase 1; PARP-1; ADP-ribosyltransferase diphtheria toxin-like 1; ARTD1; NAD (+) ADP-ribosyltransferase 1; ADPRT 1; Poly[ADP-ribose] synthase 1
Swiss Prot
P09874
Reactivity
Human
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human PARP1. The exact sequence is proprietary.
Conjugation
Unconjugated
Related Pathways
NF-kB Signaling, Death Receptor Signaling
Applications
WB, IHC
Dilution
WB (1/1000 - 1/3000), IHC (1/200 - 1/500)
Purity
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 116 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of PARP1 protein.
Applications Notes
Western blot analysis of PARP1 expression in Hela (A), 293T (B), Jurkat (C) whole cell lysates.
Host or Source
Mouse
Available Sizes
Frequently Asked Questions
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