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Histone H2B monoclonal antibody

Product Specifications

Background

Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f

Swiss Prot

P33778

Reactivity

Human, Mouse, Rat

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence of human Histone H2B. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB, IHC

Dilution

WB (1/1000 - 1/3000), IHC (1/200 - 1/500)

Purity

The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 14 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of Histone H2B protein.

Applications Notes

Western blot analysis of Histone H2B expression in Hela (A), NIH3T3 (B), Raw264.7 (C), rat brain (D), rat kidney (E) whole cell lysates.

Host or Source

Mouse

Available Sizes

Frequently Asked Questions

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