Caspase 8 monoclonal antibody
Product Specifications
Background
Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3) . Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.
CAS Number
9007-83-4
Structure Composition
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
MCH5; Caspase-8; CASP-8; Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE; ICE-like apoptotic protease 5; MORT1-associated ced-3 homolog; MACH
Swiss Prot
Q14790
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein corresponding to human Caspase 8.
Conjugation
Unconjugated
Related Pathways
Regulation of Apoptosis, Necrotic Cell Death, Inhibition of Apoptosis, Death Receptor Signaling
Applications
WB, IHC
Dilution
WB (1/1000 - 1/2000), IHC (1/200 - 1/500)
Purity
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 57 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of Caspase 8 protein.
Applications Notes
Western blot analysis of Caspase 8 expression in Hela (A), mouse brain (B), rat brain (C) whole cell lysates.
Host or Source
Mouse
Available Sizes
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