Beta-catenin monoclonal antibody
Product Specifications
Background
β-catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.
CAS Number
9007-83-4
Structure Composition
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
CTNNB; Catenin beta-1; Beta-catenin
Swiss Prot
P35222
Reactivity
Human, Mouse, Rat
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence of human Beta-catenin. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB, IHC
Dilution
WB (1/1000 - 1/2000), IHC (1/100 - 1/200)
Purity
The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 92 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of Beta-catenin protein.
Applications Notes
Western blot analysis of Beta-catenin expression in Hela (A), 293T (B), MCF7 (C), mouse brain (D), rat liver (E) whole cell lysates.
Host or Source
Mouse
Available Sizes
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