GST-tag polyclonal antibody
Product Specifications
Background
GST (Glutathione S-Transferase) is a 26kDa protein encoded by the parasitic helminth Schistosoma japonicum and widely used in the pGEX family of GST plasmid expression vectors as a fusion protein with foreign proteins.
CAS Number
9007-83-4
Structure Composition
Liquid in PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.
Immunogen
Recombinant full length protein.
Conjugation
Unconjugated
Applications
ELISA, WB
Dilution
WB (1/500 - 1/1000)
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes the GST-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Applications Notes
Western blot analysis of Anti-GST-tag Antibody against HEK293T cells transfected with vector overexpressing GST tag (A) and untransfected (B) .
Host or Source
Rabbit
Available Sizes
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