Human Ig light chain Lambda monoclonal antibody
Product Specifications
Background
The immunoglobulin light chain is the smaller subunit of an antibody and in humans can be expressed in two types: as a kappa (kappa) or a lambda (lambda) chain. Antibodies are produced by B-Cells that are clonal and each expresses only one type of light chain. The light chain class remains fixed for the life of the B lymphocyte. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays.
CAS Number
9007-83-4
Structure Composition
Mouse IgG1. Liquid in PBS, pH 7.3, and 0.02% sodium azide.
Reactivity
Human
Immunogen
Native purified human Ig light chain Lambda.
Conjugation
Unconjugated
Applications
IF, FC
Dilution
IF (1/50 - 1/200)
Purity
The monoclonal antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 25 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes human Ig light chain Lambda
Applications Notes
Flow cytometric analysis of human peripheral blood lymphocytes using Anti-Ig light chain Lambda Antibody, followed by anti-mouse IgG PE.
Host or Source
Mouse
Available Sizes
Curated Selection
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