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CDKAL1 polyclonal antibody

Product Specifications

Background

Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t6A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms2t6A) at position 37 in tRNAs that read codons beginning with adenine.

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

Threonylcarbamoyladenosine tRNA methylthiotransferase; CDK5 regulatory subunit-associated protein 1-like 1; tRNA-t (6) A37 methylthiotransferase

Swiss Prot

Q5VV42

Reactivity

Human, Rat, Mouse

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human CDKAL1. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB

Dilution

WB (1/500 - 1/1000)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 184% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of CDKAL1 protein.

Applications Notes

Western blot analysis of CDKAL1 expression in HEK293T (A), MCF7 (B), H1792 (C), mouse muscle (D), mouse spleen (E), rat muscle (F), rat spleen (G) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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