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Kir5.1 (phospho-S416) polyclonal antibody

Product Specifications

Background

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance. In the kidney, together with KCNJ10, mediates basolateral K+ recycling in distal tubules; this process is critical for Na+ reabsorption at the tubules (PubMed:24561201) .

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

KCNJ16; Inward rectifier potassium channel 16; Inward rectifier K (+) channel Kir5.1; Potassium channel inwardly rectifying subfamily J member 16

Swiss Prot

Q9NPI9

Reactivity

Human, Rat, Mouse

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Kir5.1 with a phosphorylation site at S416. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB, IHC, IF/IC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200), IF/ICC (1/50 - 1/200)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 156% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 55 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of Kir5 protein.1 with a phosphorylation site at S416 protein.

Applications Notes

Western blot analysis of Kir5.1 (pS416) expression in HEK293T (A), HuT78 (B), mouse spleen (C), rat spleen (D) whole cell lysates.

Host or Source

Rabbit

Available Sizes

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