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USP51 polyclonal antibody

Product Specifications

Background

Specifically deubiquitinates 'Lys-14' (H2AK13Ub) and 'Lys-16' (H2AK15Ub) of histone H2A regulating the DNA damage response at double-strand breaks (DSBs) (PubMed:27083998) . USP51 is recruited to chromatin after DNA damage and regulates the dynamic assembly/disassembly of TP53BP1 and BRCA1. Exhibits also activity for 'Lys-27' or 'Lys-63'-linked di-ubiquitin (PubMed:27083998) .

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

Ubiquitin carboxyl-terminal hydrolase 51; Deubiquitinating enzyme 51; Ubiquitin thioesterase 51; Ubiquitin-specific-processing protease 51

Swiss Prot

Q70EK9

Reactivity

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human USP51. The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB, IHC

Dilution

WB (1/500 - 1/1000), IHC (1/50 - 1/200)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 102% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 80 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of USP51 protein.

Applications Notes

Western blot analysis of USP51 expression in CT26 (A), A549 (B), EC9706 (C) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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