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B-RAF (phospho-S446) polyclonal antibody

Product Specifications

Background

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable) . Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126) .May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179) .

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1

Swiss Prot

P15056

Reactivity

Human, Mouse, Rat

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human B-RAF. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

MAPK Erk in Growth and Differentiation Pathway

Applications

WB, IHC

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 93 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of B-RAF (pS446) protein.

Applications Notes

Western blot analysis of B-RAF (pS446) expression in HEK293T (A), mouse brain (B), rat brain (C) whole cell lysates.

Host or Source

Rabbit

CAS Number

9007-83-4

Available Sizes

Curated Selection

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