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Ezrin/Radixin/Moesin (phospho-T567/564/558) polyclonal antibody

Product Specifications

Background

Ezrin-radixin-moesin (ERM) family protein that connects the actin cytoskeleton to the plasma membrane and thereby regulates the structure and function of specific domains of the cell cortex. Tethers actin filaments by oscillating between a resting and an activated state providing transient interactions between moesin and the actin cytoskeleton (PubMed:10212266) .

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

MSN; Moesin; Membrane-organizing extension spike protein; RDX; Radixin; EZR; VIL2; Ezrin; Cytovillin; Villin-2; p81

Swiss Prot

P26038; P35241; P15311

Reactivity

Human, Mouse, Rat

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Ezrin/Radixin/Moesin . The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 78 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of Ezrin/Radixin/Moesin (pT567/564/558) protein.

Applications Notes

Western blot analysis of Ezrin/Radixin/Moesin (pT567/564/558) expression in SKOV3 (A), NIH3T3 (B) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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