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WASP (phospho-Y290) polyclonal antibody

Product Specifications

Background

Effector protein for Rho-type GTPases that regulates actin filament reorganization via its interaction with the Arp2/3 complex (PubMed:12235133, PubMed:12769847, PubMed:16275905) .Important for efficient actin polymerization (PubMed:8625410, PubMed:12235133, PubMed:16275905) .Possible regulator of lymphocyte and platelet function (PubMed:9405671) .

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

IMD2; Wiskott-Aldrich syndrome protein; WASp

Swiss Prot

P42768

Reactivity

Human, Mouse, Rat

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human WASP (pY290) . The exact sequence is proprietary.

Conjugation

Unconjugated

Applications

WB, IHC, IF/ICC, FC

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500), FC (1/100 - 1/200)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Phosphorylation

Molecular Weight

~ 70 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of WASP (pY290) protein.

Applications Notes

Western blot analysis of WASP (pY290) expression in Jurkat (A), SHSY5Y (B), mouse spleen (C) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Curated Selection

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