ULK3 polyclonal antibody
Product Specifications
Background
Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3) . Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.
CAS Number
9007-83-4
Structure Composition
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
Serine/threonine-protein kinase ULK3; Unc-51-like kinase 3
Swiss Prot
Q6PHR2
Reactivity
Human, Mouse, Rat
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ULK3. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB, IHC
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 53 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of ULK3 protein.
Applications Notes
Western blot analysis of ULK3 expression in PC12 (A), COS7 (B), MCF7 (C) whole cell lysates.
Host or Source
Rabbit
Available Sizes
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