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RagA/B polyclonal antibody

Product Specifications

Background

Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade (PubMed:20381137, PubMed:24095279, PubMed:25936802) .

CAS Number

9007-83-4

Structure Composition

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Product Name Alternative

RRAGA; Ras-related GTP-binding protein A; Rag A; RagA; Adenovirus E3 14.7 kDa-interacting protein 1; FIP-1; RRAGB; Ras-related GTP-binding protein B; Rag B; RagB

Swiss Prot

Q7L523; Q5VZM2

Reactivity

Human, Mouse, Rat

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RagA/B. The exact sequence is proprietary.

Conjugation

Unconjugated

Related Pathways

Translational Contral elF4 and p70 S6 Kinase, MTOR signaling

Applications

WB, IHC

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/200)

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 35 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Recognizes endogenous levels of RagA/B protein.

Applications Notes

Western blot analysis of RagA/B expression in mouse lung (A), rat testis (B) whole cell lysates.

Host or Source

Rabbit

Available Sizes

Frequently Asked Questions

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