MRPL16 polyclonal antibody
Product Specifications
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein.
CAS Number
9007-83-4
Structure Composition
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Product Name Alternative
39S ribosomal protein L16 mitochondrial; L16mt; MRP-L16
Swiss Prot
Q9NX20
Reactivity
Human
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MRPL16. The exact sequence is proprietary.
Conjugation
Unconjugated
Applications
WB, IHC
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/200)
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 28 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
Recognizes endogenous levels of MRPL16 protein.
Applications Notes
Western blot analysis of MRPL16 expression in HEK293T (A), A549 (B) whole cell lysates.
Host or Source
Rabbit
Available Sizes
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