WT1 polyclonal antibody
Product Specifications
Background
Wilms' tumor (WT) is an embryonal malignancy of the kidney that affects 1 in 10,000 infants and, like retinoblastoma, is observed in both sporadic and inherited forms. The Wilms' tumor locus has been mapped at chromosome 11p13 as a tumor suppressor gene which encodes a DNA binding protein with four zinc fingers and a glutamine-proline rich amino-terminus. The Wilms' tumor protein (WT1) binds the DNA sequence GCGGGGGCG, a recognition element common to the early growth response (Egr) family of Zn2+ finger transcriptional activators. However, in contrast to Egr transcription factors, WT1 behaves as a transcriptional repressor in transient transfection assays with synthetic promotor constructs.
CAS Number
9007-83-4
Structure Composition
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Product Name Alternative
WIT 2; WT 1; AWT1; FWT1; GUD; NPHS4; WAGR; Wilms tumor 1; Wilms Tumor; Wilms tumor protein; Wilms' tumor gene; Wilms' tumor protein; WIT2; WT; WT1; WT-1; WT1_HUMAN; WT33
Swiss Prot
P19544
Reactivity
Human, Mouse, Rat
Immunogen
KLH conjugated synthetic peptide derived from human WT1: 301-400/449
Conjugation
Unconjugated
Applications
WB IF IHC ELISA
Dilution
WB: 1:500~1:2000 IHC: 1:100~1:500 IF: 1:100~1:500 ELISA: 1:5000~1:10000
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .
Modification
Unmodification
Molecular Weight
~ 48 kDa
Storage Conditions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Notes
For research use only, not for use in diagnostic procedure.
Specificity
WT1 polyclonal antibody detects endogenous levels of WT1 protein.
Applications Notes
Sample: TM4 Cell (Mouse) Lysate at 40 ug Primary: Anti-WT1 at 1/300 dilution
Host or Source
Rabbit
Available Sizes
Curated Selection
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