HIF-1α (K377) polyclonal antibody

Product Specifications

Background

Cell growth and viability is compromised by oxygen deprivation (hypoxia) . Hypoxia-inducible factors, including HIF-1α, Arnt 1 (also designated HIF-1β), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Product Name Alternative

Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS domain-containing protein 8; HIF1A; BHLHE78, MOP1

Swiss Prot

Q16665

Reactivity

Human, Rat, Mouse

Immunogen

Synthetic peptide, corresponding to Human HIF-1α.

Conjugation

Unconjugated

Related Pathways

Hypoxia Signaling, Warburg Effect, Glutamine Metabolism, MTOR signaling

Applications

WB IP

Dilution

WB: 1:1000~1:2000 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 120 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

HIF-1α (K377) polyclonal antibody detects endogenous levels of HIF-1α protein.

Applications Notes

Western blot (WB) analysis of HIF-1α (K377) pAb at 1:1000 dilution Lane1:HEK293T whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:EC9706 whole cell lysate (40ug)