Caspase-9 (T125) polyclonal antibody

Product Specifications

Background

Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response. Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis.

CAS Number

9007-83-4

Structure Composition

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Product Name Alternative

CASP-9; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; APAF-3; ICE-like apoptotic protease 6; ICE-LAP6; Caspase-9 subunit p35; Caspase-9 subunit p10

Swiss Prot

P55211

Reactivity

Human

Immunogen

Synthetic peptide, corresponding to Human Caspase-9.

Conjugation

Unconjugated

Related Pathways

Regulation of Apoptosis, Inhibition of Apoptosis, Mitochondrial Control of Apoptosis, Death Receptor Signaling

Applications

WB IP

Dilution

WB: 1:1000~1:2000 IP: 1:50~1:200

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Modification

Unmodification

Molecular Weight

~ 37, 40/40, 46 kDa

Storage Conditions

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Notes

For research use only, not for use in diagnostic procedure.

Specificity

Caspase-9 (T125) polyclonal antibody detects endogenous levels of Caspase-9 protein.

Applications Notes

Western blot (WB) analysis of Caspase-9 (T125) polyclonal antibody at 1:1000 dilution Lane1:Jurkat whole cell lysate (40ug) Lane2:Jurkat treated with etoposide (25μM,5 hours) whole cell lysate (40ug) Lane3:K562 whole cell lysate (40ug) Lane4:Jurkat whole cell lysate (40ug)